LAB MANUAL IN BIOCHEMISTRY IMMUNOLOGY AND BIOTECHNOLOGY BY NIGAM PDF

Lab Manual in Biochemistry, Immunology and Biotechnology by Arti Nigam, , available at Book Depository with free delivery. Get this from a library! Lab manual in biochemistry, immunology and biotechnology. [Arti Nigam; Archana Ayyagari]. Lab Manual in Biochemistry, Immunology and Biotechnology. Front Cover. Arti Nigam, Archana Ayyagari. Tata McGraw-Hill Pub., – Biochemistry –

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English View all editions and formats Summary:. To perform liver function test LFT. To carry out chromatographic separation of the components of the given amino acid mixture using thin layer chromatography. To detect syphilis spirochaete using indirect fluorescent antibody IFA technique. To perform nogam immunosorbent assay ELISA by i direct, ii indirect, and iii sandwich methods.

To estimate ascorbic acid by titration using 2,6 – dichlorophenol indophenol. To carry out ligation of a foreign insert to the vector pBR Preview this item Preview this item. To study the effect of alkaline phosphatase treatment on plasmid DNA on ligation efficiency. Looking for beautiful books? To purify the amylase present in the crude preparation following ion-exchange chromatography. To estimate glucose-oxidase activity in the given sample.

To detect pregnancy using commercially available kits. To estimate serum cholesterol of the given sample by i Liebermann-Burchard method, ii Salkowski reaction and iii Cholesterol oxidase activity. To isolate glutamic acid from gluten of wheat flour. To resolve the given mixture of proteins manuwl native polyacrylamide gel electrophoresis PAGE.

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To purify IgG from serum sample by using the technique of affinity chromatography and confirm the manul by double immunodiffusion. To measure pH of tap water and distilled water using pH paper strips and a pH meter.

To perform fatty acid titration. To determine quantitatively the proteins present in a sample by Bradford method. To use commercially available readymade strips for the detection of glucose in the given urine sample. Account Options Sign in.

To purify the plasmid DNA obtained by: To carry out extraction and estimation of serum triglycerides.

Lab manual in biochemistry, immunology and biotechnology

To cultivate lambda phage ‘ using an appropriate E. To estimate hemoglobin content of the given blood sample using i Sahli’s hemoglobinometer; ii colorimetric method. To carry out qualitative tests for the presence of proteins in the given sample. To perform Seliwanoff’s test for detection of ketose sugars.

To determine the blood group of given blood sample by slide agglutination. To estimate Immnuology quantitatively using orcinol reagent. To resolve various lipid components in the given mixture by using gas liquid chromatography GLC.

Lab Manual in Biochemistry, Immunology and Biotechnology

To estimate thiamine in the given food sample. To perform double immunodiffusion Biocchemistry by Ouchterlony’s method. Linked Data More info about Linked Data. However, formatting rules can vary widely between applications jigam fields of interest or study.

To carry out routine analysis of the given urine sample for i physical appearance ii chemical tests. To estimate riboflavin in the given food sample. To separate a mixture of sugars glucose and maltose by thin layer chromatography.

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Suitable for undergraduate and postgraduate students in life science and allied fields, qnd book covers fundamental exercises as well as advanced protocols, along with authentic explanation of various techniques and precautions pertaining to common errors in the laboratory.

You already recently rated this item. The book covers fundamental exercises as well as advanced protocols, along with authentic explanation of various techniques and precautions pertaining to common errors in the To carry out serological examination for diagnosis of syphilis.

The book covers fundamental exercises as well as advanced protocols, along with authentic explanation of various techniques and precautions pertaining to common errors in the laboratory.

To determine kinetic properties of amylase. To estimate acid phosphatase activity and to obtain the progress curve of the enzyme.

Lab Manual in Biochemistry, Immunology and Biotechnology by Archana Ayyagari,Arti Nigam –

Please enter your name. To demonstrate the presence of cholesterol and its esters microscopically by Schultz’s method. To perform Fehling’s test to detect reducing sugars. To estimate niacin lba the given sample of foodstuff. To carry out Western blot analysis for a specific protein.

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